other:Article Title: Influencing factors in the satellitism test of Haemophilus influenzae and Haemophilus parainfluenzae supplemented V factor by Staphylococcus aureus
Article Snippet: Page 6/10 Table 2 Results of the satellitism test of 114 clinical isolates of Haemophilus spp. on different media Media S. aureus Media for activating S. aureus No. of negative results of H. in uenzae * (47) No. of positive results of H. parain uenzae ** (67) MHAPs (OXOID) ATCC 29213 BAPs 2 41 ATCC 25923 BAPs 6 41 ATCC 29213 NAPs 27 41 ATCC 25923 NAPs 32 41 MHAPs (BD) ATCC 29213 BAPs 2 42 ATCC 25923 BAPs 5 42 ATCC 29213 NAPs 26 42 ATCC 25923 NAPs 30 42 NAPs ATCC 29213 BAPs 12 55 ATCC 25923 BAPs 24 55 ATCC 29213 NAPs 30 55 ATCC 25923 NAPs 33 55 TSAPs (BD) ATCC 29213 BAPs 15 67*** ATCC 25923 BAPs 17 67*** ATCC 29213 NAPs 40 67*** ATCC 25923 NAPs 46 67*** TSAPs (BD) Supplemented with beef extract ATCC 29213 BAPs 6 67 ATCC 25923 BAPs 7 67 ATCC 29213 NAPs 29 67 ATCC 25923 NAPs 31 67 TSAPs (Huankai) ATCC 29213 BAPs 17 67 ATCC 25923 BAPs 20 67 Page 7/10 Media S. aureus Media for activating S. aureus No. of negative results of H. in uenzae * (47) No. of positive results of H. parain uenzae ** (67) ATCC 29213 NAPs 43 67 ATCC 25923 NAPs 47 67 TSAPs (Huankai) Supplemented with beef extract ATCC 29213 BAPs 8 67 ATCC 25923 BAPs 10 67 ATCC 29213 NAPs 31 67 ATCC 25923 NAPs 38 67 * Negative results: few or no satellite colonies ** Positive results: more or many satellite colonies *** One of them had relatively few satellite colonies Discussion There are two methods of the satellitism test of Haemophilus spp., one is to use discs containing V factor, X factor or both, and the other is to use S. aureus to provide V factor, and to use blood in the medium containing blood to provide X factor.
Article Title: Influencing factors in the satellitism test of Haemophilus influenzae and Haemophilus parainfluenzae supplemented V factor by Staphylococcus aureus
Article Snippet: Page 4/10 Table 1 Results of the satellitism test of six reference strains on different media* Media S. aureus Media for activating S. aureus H. in uenzae (ATCC 10211) H. in uenzae (ATCC 9766) H. in uenzae (ATCC 49247) H. In uenzae (ATCC 19418) H. parain uenzae (ATCC 9796) H. parain uenzae (ATCC 33392) MHAPs (OXOID) ATCC 29213 BAPs ++ + + - ++ ++ ATCC 25923 BAPs ++ + + - ++ ++ ATCC 29213 NAPs - - - - ++ ++ ATCC 25923 NAPs ± - - - ++ ++ MHAPs (BD) ATCC 29213 BAPs ++ + ++ - ++ ++ ATCC 25923 BAPs ++ + + - ++ ++ ATCC 29213 NAPs - - - - ++ ++ ATCC 25923 NAPs ± - - - ++ ++ NAPs ATCC 29213 BAPs + + + + - ++ ATCC 25923 BAPs + ± ± ± - ++ ATCC 29213 NAPs + ± ± ± - ++ ATCC 25923 NAPs + ± ± ± - ++ TSAPs (BD) ATCC 29213 BAPs ++ ++ + + ++ ++ ATCC 25923 BAPs ++ ++ + ± ++ ++ ATCC 29213 NAPs + ± - - ++ ++ ATCC 25923 NAPs ± - - ± ++ ++ TSAPs (BD) Supplemented with beef extract ATCC 29213 BAPs ++ ++ ++ + ++ ++ ATCC 25923 BAPs ++ ++ + ± ++ ++ ATCC 29213 NAPs + ± ± ± ++ ++ ATCC 25923 NAPs + - - - ++ ++ TSAPs (Huankai) ATCC 29213 BAPs ++ + ± - ++ ++ *-: no or almost no satellite colonies, ±: very few satellite colonies, +: more satellite colonies, ++: many satellite colonies Page 5/10 Media S. aureus Media for activating S. aureus H. in uenzae (ATCC 10211) H. in uenzae (ATCC 9766) H. in uenzae (ATCC 49247) H. In uenzae (ATCC 19418) H. parain uenzae (ATCC 9796) H. parain uenzae (ATCC 33392) ATCC 25923 BAPs ++ + ± - ++ ++ ATCC 29213 NAPs + ± ± ± ++ ++ ATCC 25923 NAPs - - - - ++ ++ TSAPs (Huankai) Supplemented with beef extract ATCC 29213 BAPs ++ + + + ++ ++ ATCC 25923 BAPs ++ + + - ++ ++ ATCC 29213 NAPs + + ± ± ++ ++ ATCC 25923 NAPs ± - - - ++ ++ *-: no or almost no satellite colonies, ±: very few satellite colonies, +: more satellite colonies, ++: many satellite colonies Satellitism test of clinical isolates The results (Table 2) of the clinical isolates were similar to those of the reference strains, except that some of the H. parain uenzae isolates did not grow or grew more poorly on MHAPs than on NAPs.
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